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Journal of IMAB - Annual Proceeding (Scientific Papers)
Publisher: Peytchinski, Gospodin Iliev
ISSN: 1312-773X (Online)
Issue: 2009, Volume 15, book 2
Subject Collection: Medicine
Page: 79-83
DOI: 10.5272/jimab.1522009_79
Online date: September 11, 2009,
In vitro study of bactericidal effect of antimicrobial agents used in modern endodontics
V. Kalchinov*, Slavcho Dimitrov*, M. Belcheva**
* Department of Conservative Dentistry, ** Department of Image and Oral Diagnostic; Faculty of Dental Medicine, Medical University - Sofia, Bulgaria
Summary:
The purpose of this study is to carry out comparative in vitro study of bactericidal effect of antimicrobial drugs used in modern endodontics against some basic microbial agents of periodontitis. Using the agar diffusion method (experiment with sterilized disks and wells) antimicrobial effect of five antimicrobial agents used in endodontics is evaluated: Rockle, Cresophene, I2/KI, Cupral, Ca(OH)2- high dispersion. For the study three types of nutritious media are used: BHI- agar, MH- agar, Columbi blood agar. Tested microorganisms are: E. faecalis, S. aureus, bacterium of Prevotella spp. We found out through the standard agar diffusion method with disks that I2/KI has biggest bactericidal effect except for two cases when Cresophene is present. Using the method with wells we studied the antimicrobial effect only for Cupral and Са(ОН)2- high dispersion. These agents have almost the same antimicrobial activity except for two cases when Cupral has bigger antimicrobial activity.
Key words: endodontics microbiology, agar diffusion method.
Page: 79-83; FULL TEXT PDF (197 KB)