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Journal of IMAB - Annual Proceeding (Scientific Papers)
Publisher: Peytchinski, Gospodin Iliev
ISSN: 1312-773X (Online)
Issue: 2009, Volume 15, book 2
Subject Collection: Medicine
Page: 28 - 31
DOI: 10.5272/jimab.1522009_28
Online date: June 25, 2009,
Effect of laser irradiation with different wavelength on the proliferation activity of human pulp fibroblast cells, depending on irradiation parameters and hard tissue thickness
Sl. Dimitrov, Violeta Dogandzhiyska, N. Ishkitiev*
Department of Operative dentistry and Endodontics, Faculty of Dental Medicin-Sofia, *Department of Biochemistry, Faculty of Medicine, Medical University, Sofia, Bulgaria
Summary:
Several studies demonstrate, that low level laser therapy /LLLT/ improve the prognosis on direct and indirect pulp capping /6, 9, 10, 11, 12, 13, 14/. Biostimulatory effect of laser irradiation represents a set of structural, biochemical and functional changes in living microorganisms /6/. The positive effect is due to unspecific stimulatory action of laser beam - increase of colagen production, enzyme activity, micro- and lymph-circulation, fibroblast proliferation, decrease of local hypoxia, antiinflammatory effect and pain reduction /3, 4, 6, 8/.
Especially strong stimulatory effect is determined by irradiation with monochromatic red and infrared light with 630-905 nm wavelength. The low level laser irradiation from red and near infrared light spectrum correspond exactly to relevant characteristic energy and absorbtion level in the respiratory chain. It acts directly on stimulating components of the so-called antenna pigments of the respiratory chain and manifest as an immediate effect cell vitalization by ATP mitochondrial production increase /3/.
Biostimulatory effect of laser irradiation is determined by the magnitude of the absorbed light energy. Energy depth of penetration depends on many factors – wave length, optical and temperature characteristics, power, energy values, exposure time, wave shape, and optical characteristics of tissue- absorbtion and scattering coefficient /1, 2, 7, 16/. Each tissue because of their structural and biochemical diversity will have individual translucence for laser irradiation with different wavelength /2/.Greater oversight is typical of beam from red spectral area, and minimal such- of beam from blue spectral area /6/. As much translucenter tissue is, as minimal absorbtion and weaker biological effect of laser irradiation is. The cement is most transparent dental hard tissue, the second is dentin. Oversight of light energy through dentin is 0,2-0,8 %, and through enamel is 0,5-1,5 % /6/.
Key words: laser irradiation, pulp fibroblast cells, proliferation, biostimulatory effect.
Page: 28-31; FULL TEXT PDF (337 KB)