J of IMAB. 2005; 11(1):3-6
COMPARISON OF THE SENSITIVITY OF THE POLIMERASE CHAIN REACTION FOR DETECTION OF CHLAMYDIA PNEUMONIAE INFECTIONS AFTER TWO DIFFERENT APPLIED METHODS FOR DNA EXTRACTION FROM SPUTA, TRACHEAL AND BRONCHIAL SECRETS
Vesela Ouzounova, Ivan Mitov
Department of Microbiology, Medical Faculty, Medical University-Sofia, Bulgaria.

ABSTRACT:
Chlamydia pneumoniae (Chlamydophila pneumoniae) is one of the important pathogens in human respiratory tract infections. The microogranism is an obligatory intracellular gram-negative bacterium that is common cause of bronchitis, sinusitis, pharyngitis and atypical pneumonia. 90% or more of C. pneumoniae infections remain asymptomatic. There is suggested a relationship between C. pneumoniae and chronic diseases such as chronic obstructive pulmonary disease, coronary heart disease, multiple sclerosis, reactive arthritis, Guillain-Barre’ syndrome, Alzheimer’s disease, etc. The knowledge for the epidemiology of C. pneumoniae infections mainly depends on serological detection received from studies of the population immune status using microimmunofluorescence and cell culture. These methods are time consuming and not very sensitive. Recently the isolation of the microorganism was improved and more frequently the PCR started to be applied as a method of choice to diagnose C. pneumoniae infections. The method is performed within several hours and gives the possibility to use a wide variety of patient’s specimens.
The aim of this study is to compare the sensitivity of the polimerase chain reaction after two different types of DNA extraction from sputa, tracheal and bronchial secrets, artificially infected with C. pneumoniae..

Key words: Chlamydia pneumoniae, PCR, phenol/chlorophorm/isoamyl alcohol isolation, sputum, tracheal and bronchial secrets,

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Please cite this article as: Ouzounova V, Mitov I. COMPARISON OF THE SENSITIVITY OF THE POLIMERASE CHAIN REACTION FOR DETECTION OF CHLAMYDIA PNEUMONIAE INFECTIONS AFTER TWO DIFFERENT APPLIED METHODS FOR DNA EXTRACTION FROM SPUTA, TRACHEAL AND BRONCHIAL SECRETS. J of IMAB. 2005; 11(1):3-6; [Crossref -10.5272/jimab.2005111.3]

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