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Publisher: Peytchinski Publishing Ltd.
ISSN: 1312-773X (Online)
Issue: 2026, vol. 32, issue2
Subject Area: Dental Medicine
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DOI: 10.5272/jimab.2026322.6853
Published online: 12 May 2026
Cases report
J of IMAB. 2026 Apr-Jun;32(2):6853-6859
DIRECT PULP CAPPING OF DENTAL PULP WITH DIFFERENT TYPES OF PRF PLATELET CONCENTRATES -PROTOCOLS, QUALITIES, CLINICAL CASES
Janet Kirilova1
, Dimitar Yovchev2,
1) Department of Conservative Dentistry, Faculty of Dental Medicine, Medical University, Sofia, Bulgaria.
2) Department of Imaging and Oral Diagnostics, Faculty of Dental Medicine, Medical University, Sofia, Bulgaria.
ABSTRACT:
Introduction: Preservation of dental pulp vitality through direct pulp capping with platelet-rich fibrin (PRF), an autologous platelet concentrate (APC), has been demonstrated in clinical studies and case follow-up reports.
Aim: The aim of the present article was to present the clinical outcomes of vital pulp therapy using direct pulp capping with two different autologous platelet concentrate-derived membranes, namely A-PRF+ and a combination of H-PRF and e-PRF, in teeth diagnosed with reversible pulpitis and pulp exposure < 2.5 mm.
Materials and Methods: In Case 1, an A-PRF+ membrane was prepared using a centrifugation protocol in a vertical centrifuge. In Case 2, H-PRF and e-PRF membranes were prepared using centrifugation protocols in a horizontal centrifuge. In both cases, teeth diagnosed with reversible pulpitis and pulp exposure (<2.5 mm) were treated. Disinfection of the lesion was with ozone gas for 18 s, following direct pulp capping with a PRF membrane. Clinical and radiographic follow-up examinations, including cone-beam computed tomography (CBCT), were performed for up to 12 months after treatment.
Results: Both cases demonstrated successful preservation of pulp vitality, confirmed by electric pulp testing (EPT), and formation of reparative dentin, as assessed by CBCT, with measured thicknesses of 0.57 mm and 0.63 mm.
Conclusions: The use of autologous platelet concentrate-derived membranes, including A-PRF+ and a combination of H-PRF and e-PRF, for direct pulp capping in teeth with reversible pulpitis and pulp exposure < 2.5 mm was associated with maintenance of pulp vitality throughout the 12-month follow-up period and with dentin bridge formation.
Keywords: autologous platelet concentrates, PRF, A-PRF+, H-PRF, e-PRF, reversible pulpitis, vital pulp therapy, direct pulp capping,
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Please cite this article as: Kirilova J, Yovchev D. Direct Pulp Capping of Dental Pulp with Different Types of PRF Platelet Concentrates -Protocols, Qualities, Clinical Cases. J of IMAB. 2026 Apr-Jun;32(2):6853-6859. [Crossref - 10.5272/jimab.2026322.6853]
Correspondence to: Janet Kirilova, Department of Conservative Dentistry, Faculty of Dental Medicine, Medical University, Sofia; 1, Georgi Sofiiski Str., Sofia, Bulgaria; E-mail: janetkirilova@gmail.com
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Received: 02 October 2025
Published online: 12 May 2026