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head JofIMAB
Journal of IMAB - Annual Proceeding (Scientific Papers)
Publisher: Peytchinski Publishing Ltd.
ISSN: 1312-773X (Online)
Issue: 2024, vol. 30, issue1
Subject Area: Dental Medicine
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DOI: 10.5272/jimab.2024301.5356
Published online: 08 February 2024

Original article
J of IMAB. 2024 Jan-Mar;30(1):5356-5360
SPECIES CHARACTERISTICS OF MICROBIAL ASSOCIATIONS IN THE COURSE OF TREATMENT OF 10 TO 14-YEAR-OLD CHILDREN WITH PLAQUE-INDUCED GINGIVITIS
Hristina TankovaORCID logoCorresponding Autoremail, Zornitsa LazarovaORCID logo,
Department of Pediatric Dentistry, Faculty of Dental Medicine, Medical University – Sofia, Bulgaria.

ABSTRACT:
Aim: Еvaluation of the species characteristics of subgingival biofilm microbial associations during treatment of children with plaque-induced gingivitis aged 10-14 years.
Materials and methods: The subject of the study were 34 children with plaque-induced gingivitis. Three samples of subgingival biofilm are taken per patient: 1st sample - at the first visit; 2nd sample - two weeks later; 3rd  the sample - one month later. Five teeth per child are selected from which a sample is taken for PCR testing for 9 control strains of subgingival microorganisms (A. actinomycetemcomitans, P. gingivalis, T. denticola, T. forsythia, P. intermedia, P. micros, F. nuleatum, E. nodatum, C. gingivalis).
Results: C. gingivalis (green complex) is isolated in all examined children before the start of treatment and is indispensably observed in microbial associations with P. intermedia (orange complex). In other cases, associations with 7-8 microorganisms are observed and in a more complex composition. One month after the start of treatment, no periodontopathogens or only C. gingivallis as a single microorganism was is isolated in more than 55% of the samples examined. Microbial associations are predominantly 2 to 4 microorganisms.

Keywords: microbial associations, gingivitis in children, periodontopathogens in gingivitis, Socransky complexes,

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Please cite this article as: Tankova H, Lazarova Z. Species Characteristics of Microbial Associations in the Course of Treatment of 10 to 14-year-old Children with Plaque-induced Gingivitis. J of IMAB. 2024 Jan-Mar;30(1):5356-5360. [Crossref - 10.5272/jimab.2024301.5356]

Corresponding AutorCorrespondence to: Hristina Tankova, Department of Pediatric Dentistry, Faculty of Dental Medicine, Medical University – Sofia; 1, St. Georgi Sofiisky Str., Sofia, Bulgaria; E-mail: la_svetichi@yahoo.com

REFERENCES:
1. Donlan RM, Costerton JW. Biofilms: survival mechanisms of clinically relevant microorganisms. Clin Microbiol Rev. 2002 Apr;15(2):167-93. [PubMed]
2.Löe H, Theilade E, Jensen Sb. Experimental gingivitis in man. J Periodontol (1930). 1965 May-Jun;36:177-87. [PubMed]
3.Lang NP, Bartold PM. Periodontal health. J Periodontol. 2018 Jun;89(Suppl 1):S9-S16. [PubMed]
4.Listgarten MA. Structure of the microbial flora associated with periodontal health and disease in man. A light and electron microscopic study. J Periodontol. 1976 Jan;47(1):1-18. [PubMed]
5.Listgarten MA. The structure of dental plaque. Periodontol 2000. 1994 Jun;5:52-65. [PubMed]
6.Socransky SS, Haffajee AD, Cugini MA, Smith C, Kent RL Jr. Microbial complexes in subgingival plaque. J Clin Periodontol. 1998 Feb;25(2):134-14. [PubMed]
7. de Andrade DR, Silva PA, Colombo APV, Silva-Boghossian CM. Subgingival microbiota in overweight and obese young adults with no destructive periodontal disease. J Periodontol. 2021 Oct;92(10):1410-1419. [ PubMed ]
8.Wendland N, Opydo-Szymaczek J, Mizgier M, Jarząbek-Bielecka G. Subgingival microflora in adolescent females with polycystic ovary syndrome and its association with oral hygiene, gingivitis, and selected metabolic and hormonal parameters. Clin Oral Investig. 2021 Mar;25(3):1485-1496. [PubMed]
9.Yang NY, Zhang Q, Li JL, Yang SH, Shi Q. Progression of periodontal inflammation in adolescents is associated with increased number of Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythensis, and Fusobacterium nucleatum. Int J Paediatr Dent. 2014 May;24(3):226-33. [PubMed]
10.Kakudate N, Morita M, Sugai M, Kawanami M. Systematic cognitive behavioral approach for oral hygiene instruction: a short-term study. Patient Educ Couns. 2009 Feb;74(2):191-6. [PubMed]
11.Hajishengallis G, Lamont RJ. Beyond the red complex and into more complexity: the polymicrobial synergy and dysbiosis (PSD) model of periodontal disease etiology. Mol Oral Microbiol. 2012 Dec;27(6):409-19. [PubMed]
12.Mitova N, Rashkova M, Popova Ch. Quantity, diversity and complexity of subgingival microorganisms in children with plaque-induced gingivitis. BiotechnolBiotechnolEquip. 2019 Apr 24;33(1):620-626. [Crossref]
13.Werner H, Hakeberg M, Dahlström L, Eriksson M, Sjögren P, Strandell A, et al. Psychological interventions for poor oral health: a systematic review. J Dent Res. 2016 May;95(5):506-14. [PubMed]

Received: 25 October 2023
Published online: 08 February 2024

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