head JofIMAB
Journal of IMAB - Annual Proceeding (Scientific Papers)
Publisher: Peytchinski Publishing Ltd.
ISSN: 1312-773X (Online)
Issue: 2024, vol. 30, issue1
Subject Area: Medicine
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DOI: 10.5272/jimab.2024301.5295
Published online: 09 January 2024

Original article
J of IMAB. 2024 Jan-Mar;30(1):5295-5300
DEVELOPMENT AND APPLICATION OF A METHOD FOR GENETIC DETECTION OF ENTEROBIUS VERMICULARIS IN SAMPLES OF PATIENTS WITH ENTEROBIАSIS
Eleonora Kaneva1ORCID logoCorresponding Autoremail, Nina Tsvetkova1ORCID logo, Desislava Velcheva2ORCID logo, Rumen Harizanov1ORCID logo, lskren Kaftandjiev1ORCID logo, Raina Borisova1ORCID logo, Mihaela Videnova1ORCID logo, Aleksandra lvanova1ORCID logo, Ivailo Alexiev1ORCID logo, Reneta Dimitrova1ORCID logo, Maria Pavlova1ORCID logo,
1) National Centre of Infectious and Parasitic Diseases, Department of Parasitology and Tropical Medicine, Sofia, Bulgaria.
2) MDL "Cibalab", Sofia, Bulgaria.

ABSTRACT:
Purpose. The routine diagnosis of enterobiasis consists of a microscopic examination of perianal imprints on a transparent scotch tape for the presence of parasitic eggs. However, this method has low sensitivity when the number of eggs is small. The development of highly sensitive and specific methods, such as PCR, will increase the possibilities of detecting the parasite and will enable its genetic characterization.
Material/Methods. The study was conducted from May 2022 to September 2022 and comprised 24 patients infected with Enterobius vermicularis. DNA was extracted from parasite eggs/adult worms using PureLink Genomic DNA Mini Kit (INVITROGEN), according to the manufacturer's instructions. Nested polymerase chain reaction (PCR) for detection of mitochondrial cytochrome c oxidase subunit 1 (cox1) gene was applied with two sets of primers.
Results. The developed and described method for genetic determination of Enterobius vermicularis in samples from infected individuals is suitable and applicable to improve the diagnosis of the disease, as well as for further phylogenetic research of the parasite, which is of great importance for its successful treatment and control.
Conclusions. Regardless of existing morphological diagnostic methods, genotyping studies concerning Enterobius vermicularis need to be deepened. Molecular-biological analyses increase diagnostic sensitivity, provide valuable knowledge about the geographic distribution and diversity of helminths, and allow the monitoring of therapy.

Keywords: Enterobius vermicularis, enterobiasis, nested PCR, cox1 gene,

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Please cite this article as: Kaneva E, Tsvetkova N, Velcheva D, Harizanov R, Kaftandjiev I, Borisova R, Videnova M, Ivanova A, Alexiev I, Dimitrova R, Pavlova M. Development and application of a method for genetic detection of Enterobius vermicularis in samples of patients with enterobiasis. J of IMAB. 2024 Jan-Mar;30(1):5295-5300. [Crossref - 10.5272/jimab.2024301.5295]

Corresponding AutorCorrespondence to: Eleonora Kaneva, National Centre of Infectious and Parasitic Diseases, Department of Parasitology and Tropical Medicine; 26, Yanko Sakazov Blvd., 1504 Sofia, Bulgaria; E-mail: kaneva@ncipd.org

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Received: 12 May 2023
Published online: 09 January 2024

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