head JofIMAB
Journal of IMAB - Annual Proceeding (Scientific Papers)
Publisher: Peytchinski Publishing Ltd.
ISSN: 1312-773X (Online)
Issue: 2021, vol. 27, issue2
Subject Area: Dental Medicine
DOI: 10.5272/jimab.2021272.3712
Published online: 26 April 2021

Original article

J of IMAB. 2021 Apr-Jun;27(2):3712-3717
Tzvetelina G. Gueorgieva1ORCID logo Corresponding Autoremail, Raina T. Gergova2ORCID logo,
1) Department of Conservative Dentistry, Faculty of Dental Medicine, Medical University Sofia, Bulgaria.
2) Department of Medical Microbiology, Faculty of Medicine, Medical University Sofia, Bulgaria.

Introduction: The purpose of endodontic treatment is to eliminate the bacterial infection in the root canal system and allow healing of apical periodontitis. Sometimes the anatomical complexity of the root canal system makes complete removal of bacteria almost impossible even if the conventional methods of chemo-mechanical debridement are performed strictly according to the protocol. So additional methods of root canal disinfection can be applied such as photoactivated disinfection (PAD).
Purpose: The aim of our study was to compare the antibacterial activity of PAD with FotoSan, and conventional endodontic therapy in the treatment of infected root canals.
Materials and methods: The study involved 36 teeth of patients who are diagnosed with pulp necrosis or with the periapical chronic periodontitis and require endodontic treatment and divided into two groups of 18 teeth each. The first microbiological sample was taken by placing a sterile paper point in the root canal, after endodontic access cavity preparation.
 All root canals are prepared by Protaper Universal rotary instruments (Maillefer Instruments SA, Ballaigues, Switzerland). In the first group, the disinfection of the root canals was made with photoactivated disinfection with FotoSan. In the second group was performed the endodontic treatment with the standard protocol of irrigation - 2.5% sodium hypochlorite solution and 17% EDTA. After that, the root canals are dried with sterile points, and a second microbiological sample is taken.
Results: In all compared pairs, there was no significantly different effect regarding the number of microorganisms.
Conclusions; The disinfection with NaOCl has the strongest antimicrobial effect in this study. Second place is occupied by PAD with FotoSan. We believe that the established antimicrobial effect of PAD make the method appropriate as complementary to routine endodontic treatment.

Keywords: Endodontic treatment, root canal disinfection, photoactivated disinfection,

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Please cite this article as: Gueorgieva TG, Gergova RT. Comparative study of Antibacterial Activity of photoactivated disinfection with Fotosan and Standard Endodontic Treatment. J of IMAB. 2021 Apr-Jun;27(1):3712-3717. DOI: 10.5272/jimab.2021272.3712

Corresponding AutorCorrespondence to: Dr Tzvetelina G. Gueorgieva, PhD, Department of Conservative Dentistry, Faculty of Dental Medicine, Medical University – Sofia; 1, St. George Sofiyski str., Sofia, Bulgaria; E-mail: cvettyg@gmail.com

1. Gergova R, Georgieva T, Angelov I, Mantareva V, Valkanov S, Mitov I, et al. Photodynamic therapy with water-soluble phtalocyanines against bacterial biofilms in teeth root canals. SPIE. Digital Library. 2012; 8427: Biophotonics: Photonic Solutions for Better Health Care III, 842744. [Crossref]
2. Giuliani F, Martinelli M, Cocchi A, Arbia D, Fantetti L, Roncucci G. In vitro resistance selection studies of RLP068/Cl, a new Zn(II) phthalocyanine suitable for antimicrobial photodynamic therapy. Antimicrob Agents Chemother. 2010 Feb;54(2):637-42. [PubMed]
3. Chivatxaranukul P, Dashper SG, Messer HH. Dentinal tubule invasion and adherence by Enterococcus faecalis. Int Endod J. 2008 Oct; 41(10): 873–882. [PubMed].
4. Dogandzhiyska V, Gergova R, Dimitrov S, Doychinova M. Antimicrobial activity of Photodynamic therapy against microorganisms isolated from deep carious lesions. J of IMAB. 2013 Jul-Dec;19(4):430-434. [Crossref]
5. Fonseca MB, Tessare PO Jr, Pallota RC, Filho HF, Denardin OV, Rapoport A, et al. Photodynamic therapy for root canals infected with Enterococcus faecalis. Photomed Laser Surg. 2008 Jun;26(3):209-13. [PubMed]
6. Solana C, Ruiz-Linares M, Baca P, Valderrama MJ, Arias-Moliz  MT, Ferrer-Luque CM. Antibiofilm Activity of Sodium Hypochlorite and Alkaline Tetrasodium EDTA Solutions. J Endod. 2017 Dec;43(12):2093-2096. [PubMed]
7. Gergova RT, Gueorgieva T, Dencheva-Garova MS, Krasteva-Panova AZ, Kalchinov V, Mitov I, et al. Antimicrobial activity of different disinfection methods against biofilms in root canals. J Investig Clin Dent. 2016 Aug;7(3):254-62. [PubMed]
8. Signoretti FG, Endo MS, Gomes BP, Montagner F, Tosello FB, Jacinto RC. Persistent Extraradicular Infection in Root-filled Asymptomatic Human Tooth: Scanning Electron Microscopic Analysis and Microbial Investigation after Apical Microsurgery. J Endod. 2011 Dec;37(12):1696-700. [PubMed]
9. Kuzekanani M, Plotino G, Gutmann JL. Current Applications of Lasers in Endodontics. G Ital Endod. 2019 Mar;33(02):13-23. [Crossref]
10. Matos Neto M, Santos SS, Leгo MV, Habitante SM, Rodrigues JR, Jorge AO. Effectiveness of three instrumentation systems to remove Enterococcus faecalis from root canals. Int Endod J. 2012 May;45(5):435-8. [PubMed].
11. Bago I, Plecko V, Gabric DP, Schauperl Z, Baraba A, Anic I. Antimicrobial efficacy of a high-power diode laser,photo-activated disinfection, conventional and sonic activated irrigation during root canal treatment. Int Endod J. 2013 Apr;46(4):339-47. [PubMed].
12.Alrikaby HA, Balsam SA. Comparison between the Bactericidal Effect of Nd: YAG Laser and Sodium Hypochlorite: An In vitro Study. Int J Med Res Health Sci 2018; 7(11): 49-56.
13. Bergmans L, Moisiadis P, Huybrechts B, Van Meerbeek B, Quirynen M, Lambrechts P. Effect of photo-activated disinfection on endodontic pathogens ex vivo. Int Endod J. 2008 Mar;41(3):227-39. [PubMed].

Received: 07 January 2020
Published online: 26 April 2021

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