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Publisher: Peytchinski Publishing Ltd.
ISSN: 1312-773X (Online)
Issue: 2021, vol. 27, issue1
Subject Area: Medicine
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DOI: 10.5272/jimab.2021271.3539
Published online: 22 January 2021
Original article
J of IMAB. 2021 Jan-Mar;27(1):3539-3542
COMPARATIVE ANALYSIS OF THE QUANTITATIVE AND QUALITATIVE METHOD FOR DETERMINATION OF D - DIMER
Irena I. Gencheva
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Department of Clinical Laboratory, Clinical Immunology and Allergology, Medical University - Pleven, Bulgaria.
ABSTRACT:
Introduction: D - dimer is a product released during the process of blood clotting and degradation, which can be measured by blood sample analysis. There is usually the minimal activity of the pro/anticoagulant system in the human body, which generates low levels of D-dimer in healthy individuals. Normal values for plasma D-dimer are ≤ 0.50 mg / l.
Aim: The aim of the present study is to determine to what extent the quantitative and qualitative method for determination of D - dimer can be interchangeable and what is their diagnostic reliability in the normal and pathological area of measurement.
Materials and methods: We studied the levels of D-dimer by two methods - quantitative and qualitative, in 91 patients aged 25 to 86 years, of which 59 men and 32 women. To determine the D-dimer, we used venous blood taken in a vacuette containing sodium citrate. We used a Roche test for quantitative determination and a Latex agglutination test for qualitative determination.
Results: It was found that in positive samples above 0.5 mg/l, there is a very high percentage of coincidence. There is a discrepancy in the values obtained by the two methods at the negative values below 0.5 mg/l. We determined the sensitivity, specificity and accuracy of both methods.
Conclusion: The correlation in the results of the two methods is very good, but the quantification of D-dimer is more specific and accurate. We recommend that the value of 0.5 mg/l should be used as a cut off value for D-dimer.
Keywords: D-dimer, qualitative, quantitative, cut off,
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Please cite this article as: Gencheva II. Comparative analysis of the quantitative and qualitative method for determination of D - dimer. J of IMAB. 2021 Jan-Mar;27(1):3539-3542.
DOI: 10.5272/jimab.2021271.3539
Correspondence to: Irena I. Gencheva, Department of Clinical laboratory, Clinical immunology and alergology, Medical University – Pleven; 7, Dame Gruev Str., 5800 Pleven, Bulgaria; E-mail: gencheva1677@gmail.com
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Received: 02 October 2019
Published online: 22 January 2021