head JofIMAB
Journal of IMAB - Annual Proceeding (Scientific Papers)
Publisher: Peytchinski Publishing
ISSN: 1312-773X (Online)
Issue: 2017, vol. 23, issue 4
Subject Area: Dental Medicine
DOI: 10.5272/jimab.2017234.1813
Published online: 15 December 2017

Original article

J of IMAB 2017 Oct-Dec;23(4):1813-1822
Mariana Yankova1ORCID logo Corresponding Autoremail, Bozhidar Yordanov1ORCID logo, Radoslav Baykuchev2, Vessela Raykova2, Ivan Mitov2ORCID logo,
1) Department of Prosthetic Dental Medicine, Faculty of Dental Medicine, Medical University – Sofia, Bulgaria
2) Department of Medical Microbiology, Faculty of Medicine, Medical University – Sofia, Bulgaria.

Introduction: Patients with advanced bone resorption, non-resilient mucosa or presence of exostoses, require specific methods of prosthetic treatment with complete or partial dentures, namely the use of soft materials for lining. Their porous structure is a prerequisite for bacterial and fungal colonization, that creates conditions for the occurrence of denture stomatitis.
The purpose of the present in vivo study is to explore the species and quantity of Candida spp. in the saliva of patients with complete dentures lined with two types of silicone-based elastic materials after a six-month period of observation.
Material and methods: The research was conducted upon 45 completely edentulous patients at the age from 48 to 90 (67,9 ± 9,99 years on the average). They were divided into three groups (A, B, C). For the identification and quantity assessment of the presence of Candida spp. in saliva, the researchers used Chrom agar Candida (BioMerieux) and MALDI TOF spectrophotometric analysis – VITEK MS (BioMerieux).
Results: The screening of the patients before denture delivery demonstrated absence of Candida spp. in the saliva of 44.4% and Candida albicans – 24.4%. After denture delivery, on the third and the sixth month, we found an increased quantity of C.tropicalis in the patients of groups A and B. In the saliva of the patients with denture stomatitis, the predominantly present species was C.tropicalis (54%) isolated in quantities over 104 CFU/ml.
Conclusion: The presence of C. tropicalis in the saliva related to this type of treatment is a prerequisite for the development of hard-to-treat candidoses.

Keywords: soft denture liner, resilient liner, Candida spp., saliva, denture stomatitis,

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Please cite this article in PubMed Style or AMA (American Medical Association) Style:
Yankova M, Yordanov B, Baykuchev R, Raykova V, Mitov I. Presence of Candida spp. in the saliva of patients with complete dentures, lined with silicone-based elastic materials. J of IMAB. 2017 Oct-Dec;23(4):1813-1822. DOI: 10.5272/jimab.2017234.1813

Corresponding AutorCorrespondence to: Mariana Yankova, Department of Prosthetic Dental Medicine, Faculty of Dental Medicine, Medical University, Sofia; 1, St. Georgi Sofiiski blvd., Sofia, Bulgaria; E-mail: marianayankova13@gmail.com

1. Hayakawa I. Principles and practices of complete dentures: Creating the mental image of a denture. Quintessence Pub Co. 1st edition. April 1, 1999; pp.233-248.
2. Basker RM, Davenport JC. Prosthetic Treatment of the Edentulous Patient. 16 - Some clinical problems and solution. Blackwell Publishing Company. 4th edition. 2002; pp.275-284.
3. Valentini F, Luz MS, Boscato N, Pereira-Cenci T. Biofilm formation on denture liners in a randomised controlled in situ trial. J Dent. 2013 May;41(5):420-27. [PubMed] [CrossRef]
4. Boscato N, Radavelli A, Faccio D, Loguercio AD. Biofilm formation of Candida albicans on the surface of a soft denture-lining material. Gerodontology. 2009 Sep;26(3):210-3. [PubMed] [CrossRef]
5. Budtz-Jørgensen E. Ecology of Candida-associated Denture Stomatitis. Microbial Ecology in Health and Disease. 2000 Sep; 12:170–185. [Crossref]
6. Gusmão JM, Ferreira dos Santos SS, Neisser MP, Jorge AO, Faria MI. Correlation between factors associated with the removable partial dentures use and Candida spp. in saliva. Gerodontology. 2011 Dec;28(4):283-88. [PubMed] [CrossRef]
7. Luo G., Samaranayake LP. Candida glabrata, an emerging fungal pathogen, exhibits superior relative cell surface hydrophobicity and adhesion to denture acrylic surfaces compared with Candida albicans. APMIS. 2002 Sep;110(9):601-10. [PubMed] [CrossRef]
8. Wright PS. Observations on long-term use of a soft-lining material for mandibular complete dentures. J Prosthet Dent. 1994 Oct;72(4):385-92. [PubMed] [CrossRef]
9. Nikawa H, Jin C, Hamada T. Murata H. Interactions between thermal cycled resilient denture lining materials, salivary and serum pellicles and Candida albicans in vitro. Part I. Effects on fungal growth. J Oral Rehabil. 2000 Jan;27(1):41-51. [PubMed] [CrossRef]
10. Nikawa H, Jin C, Makihira S, Egusa H, Hamada T, Kumagai H. Biofilm formation of Candida albicans on the surfaces of deteriorated soft denture lining materials caused by denture cleansers in vitro. J Oral Rehabil. 2003 Mar;30(3):243-50. [PubMed] [CrossRef]
11. Newton AV. Denture sore mouth. A possible etiology. Br Dent J. 1962; 112:357-60.
12. Jackson SL. Candida albicans biofilms in denture wearers [dissertation]. Manchester Metropolitan University. 2013 Jul;p.14.
13. Mestecky J., Strober W., Russell MW., Cheroutre H., Lambrecht BN., Kelsall BL. Mucosal immunology. Fourth edition, Elsevier 2015; pp.1518-46.
14. Paranhos Hde F, Salles AE, Macedo LD, Silva-Lovato CH, Pagnano VO, Watanabe E. Complete denture biofilm after brushing with specific denture paste, neutral soap and artificial saliva. Braz Dent J. 2013; 24(1):47-52. [PubMed] [CrossRef]
15. Gleiznys A, Zdanavičienė E, Žilinskas J. Candida albicans importance to denture wearers. A literature review. Stomatologija. 2015 Jun;17(2):54-66. [PubMed]
16. Zomorodian K, Haghighi N, Rajaee N, Pakshir K, Tarazooie B, Vojdani M, et al. Assessment of Candida species colonization and denture-related stomatitis in complete denture wearers. Med Mycol. 2011 Feb;49(2):208-11. [PubMed] [CrossRef]
17. Le Bars P, Kouadio AA, N'goran JK, Badran Z, Soueidan A.Relationship between removable prosthesis and some systemics disorders. J Indian Prosthodont Soc. 2015 Oct-Dec;15(4):292-9. [PubMed] [CrossRef]
18. Pereira-Cenci T, Del Bel Cury AA, Crielaard W, Ten Cate JM. Development of Candida-associated denture stomatitis: new insights. J Appl Oral Sci. 2008 Mar-Apr;16(2):86-94. [PubMed] [CrossRef]
19. Altarawneh S, Bencharit S, Mendoza L, Curran A, Barrow D, Barros S, et al. Clinical and histological findings of denture stomatitis as related to intraoral colonization patterns of Candida albicans, salivary flow, and dry mouth.  J Prosthodont. 2013 Jan;22(1):13-22. [PubMed] [CrossRef]
20. Abaci O, Haliki-Uztan A, Ozturk B, Toksavul S, Ulusoy M, Boyacioglu H. Determining Candida spp. incidence in denture wearers. Mycopathologia. 2010 May;169(5):365-72. [PubMed] [CrossRef]
21. Fujitani S, Cohen-Melamed MH, Tuttle RP, Delgado E, Taira Y, Darby JM. Comparison of semi-quantitative endotracheal aspirates to quantitative non-bronchoscopic bronchoalveolar lavage in diagnosing ventilator-associated pneumonia. Respir Care. 2009 Nov;54(11):1453-61. [PubMed].

Received: 26 September 2017
Published online: 15 December 2017

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