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Publisher: Peytchinski Publishing
ISSN: 1312-773X (Online)
Issue: 2017, vol. 23, issue 2
Subject Area: Dental Medicine - Prosthetic Dentistry
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DOI: 10.5272/jimab.2017232.1523
Published online: 26 April 2017
Original Article
J of IMAB 2017 Apr-Jun;23(2):1523-1526
AN OPTIMIZED GC-MS METHOD FOR IDENTIFICATION OF COCAINE AND ITS MAJOR METABOLITES
Georgi Bonchev
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Laboratory of analytical toxicology, Military Medical Academy, Naval hospital - Varna, Bulgaria.
ABSTRACT:
Purpose: To develop a simple yet sensitive, reliable and robust method for qualitative determination of cocaine and a series of its tropane metabolites in biological samples (blood, urine, etc.). The method should be optimized for routine forensic expertise in analytical toxicology.
Material/Methods: Gas chromatography coupled with mass spectrometry (GC-MS) has been used as the method for analytical identification. Certified analytical standards were used as a reference material for validation.
Results: Sample preparation procedure has been simplified and organized in such a way, that only common reagents are required. Initial matrix purification is done by means of solid phase extraction (SPE), during which a carefully chosen set of simple solvents is applied. Additional derivatization (BSTFA/MTBSTFA) has been shown to be of a major importance for benzoylecgonine determination. Limit of detection (LOD) has been determined to be as low as 50 ng/mL for cocaine and 25 ng/mL for benzoylecgonine. Results are given within 90 minutes.
Keywords: cocaine, benzoylecgonine, solid phase extraction, analytical toxicology, drugs of abuse, forensic expertise,
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Please cite this article in PubMed Style or AMA (American Medical Association) Style:
Bonchev G. An optimized GC-MS method for identification of Cocaine and its major metabolites. J of IMAB. 2017 Jan-Mar;23(2):1523-1526. DOI: 10.5272/jimab.2017232.1523
Correspondence to: Georgi Bonchev, PhD, Head, Laboratory of Analytical Toxicology, Military Medical Academy, Naval Hospital – Varna; 3, Hristo Smirnenski Blvd., 9010 Varna, Bulgaria; E-mail: toxilab.varna@abv.bg
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Received: 10 February 2017
Published online: 26 April 2017