Journal of IMAB - Annual Proceeding (Scientific Papers)
Publisher: Peytchinski, Gospodin Iliev
ISSN: 1312 773X (Online)
Issue: 2016, vol. 22, issue 4
Subject Area: Medicine
Pages: 1379-1382
DOI: 10.5272/jimab.2016224.1379
Published online: 12 December 2016

J of IMAB 2016 Oct-Dec;22(4):1379-1382
Tsvetan Sokolov1 Corresponding Autor, Aneliya Manukova2, Stefka Karakoleva3, Boyan Valentinov1, Pencho Kosev1,
1) Department of Orthopedics and Traumatology, MHAT Ruse, Ruse, Bulgaria,
2) Department of Electronics, University of Ruse, Ruse, Bulgaria,
3) Department of Applied Mathematics and Statistics, University of Ruse, Ruse, Bulgaria.

OBJECTIVE: To show platelet-rich plasma (PRP) application of problematic skin wounds and to evaluate the results from the treatment.
MATERIAL AND METHODS: A total of 31 patients with problematic skin wounds had been treated at the clinic for a period of five years (from May 2010 to September 2015) with the following patient sex ratio: male patients– 13 and female patients– 18. Average age– 46,5 (22-82). Patients with Type 2 Diabetes– 10, with decubitus ulcers– 2, traumatic– 29, with infection– 12, acute– 15, chronic– 16. Based on a scheme developed by us, all cases were treated by administering platelet-rich plasma, derived by PRGF Endoret system. Follow-up period was within 4 – 6 months (4,5 on average). We used platelet rich plasma derived by PRGF Endoret system, applied on the wound bed on a weekly basis.
RESULTS: The results have been evaluated based on the following functional scoring systems - Total wound score, Total anatomic score and Total score (20). The baseline values at the very beginning of the follow-up period were as follows: Total wound score – 10 p.; Total anatomic score – 8 p., Total score – 15 p. By the end of the treatment period the score was 0 p., which means excellent results, i.e. complete healing of the wounds.
CONCLUSION: We believe that the application of PRP may become optimal therapy in the treatment of difficult to heal wounds around joints, bone, subject tendons, plantar surface of the foot, etc., as it opens new perspectives in the field of human tissue regeneration.

Key words: problematic skin wounds, platelet rich plasma, functional scoring scales,

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Please cite this article in PubMed Style or AMA (American Medical Association) Style:
Sokolov T, Manukova A, Karakoleva S, Valentinov B, Kosev P. Platelet-rich plasma (PRP) for the treatment of problematic skin wounds. J of IMAB. 2016 Oct-Dec;22(4):1379-1382. DOI: 10.5272/jimab.2016224.1379.

Correspondence to: Dr. Tsvetan Sokolov, Department of Orthopedics and Traumatology, MHAT Ruse, Ruse, Bulgaria; 2, Nezavisimost str., 7002 Ruse, Bulgaria; E-mail:

1. Crovetti G, Martinelli G, Issi M, Barone M, Guizzardi M, Campanati B, et al. Platelet gel for healing cutaneous chronic wounds. Transfus Apher Sci. 2004 Apr;30(2):145-51. [PubMed] [CrossRef]
2. Cancela AM, Lana JF, Annichino-Bizzachi JM, Belangero WD, Luzo ACM. Use of Platelet-Rich Plasma (PRP) in Treating Chronic Wounds. In: Lana JF, et al. (eds.), Platelet-Rich Plasma. Lecture Notes in Bioengineering. Springer-Verlag Berlin, Heidelberg. 2014; p 281-288. [CrossRef].
3. Eming SA, Martin P, Tomic-Canic M. Wound repair and regeneration: mechanisms, signaling, and translation. Sci Transl Med. 2014 Dec 3;6(265):265sr6. [PubMed] [CrossRef]
4. Bielecki T, Gazdzik TS, Szczepanski T. Benefit of percutaneous injection of autologous platelet-leukocyte-rich gel in patients with delayed union and nonunion. Eur Surg Res. 2008; 40(3):289-96. [PubMed] [CrossRef]
5. Anitua E, Andia I, Ardanza B, Nurden P, Nurden AT. Autologous platelets as a source of proteins for healing and tissue regeneration. Thromb Haemost. 2004 Jan;91(1):4-15. [PubMed] [CrossRef]
6. Lana JF, Weglein A, Vicente E, Perez AG, Rodrigues A, Luzo AC, et al. Platelet Rich Plasma and Its Growth Factors: The State of the Art. In: Lana JF, et al. (eds.), Platelet-Rich Plasma. Lecture Notes in Bioengineering. Springer-Verlag Berlin, Heidelberg. 2014; p1-59. [CrossRef].
7. Cugat R. Front Matter: Foreword I. In: Lana JF, et al. (eds.), Platelet-Rich Plasma. Regenerative Medicine: Sports Medicine, Orthopedic, and Recovery of Musculoskeletal Injuries. Springer-Verlag Berlin, Heidelberg. 2014; p v-vi. [CrossRef]
8. Virchenko O, Aspenberg P. How can one platelet injection after tendon injury lead to a stronger tendon after 4 weeks? Interplay between early regeneration and mechanical stimulation. Acta Orthop. 2006 Oct;77(5):806-812. [PubMed] [CrossRef]
9. Anitua E, Aguirre JJ, Algorta J, Ayerdi E, Cabezas AI, Orive G, et al. Effectiveness of autologous preparation rich in growth factors for the treatment of chronic cutaneous ulcers. J Biomed Mater Res B Appl Biomater. 2008 Feb;84B(2):415–421. [PubMed]
10. Yuan T, Zhang CQ, Tang MJ, Guo SC, Zeng BF. Autologous platelet-rich plasma enchances healing of chronic wounds. Wounds. 2009 Oct;21(10):138-141. [PubMed]
11. Suryanarayan S, Budamakuntla L, Sha Khadri SI, Sarvajnamurthy S.Efficacy of autologous platelet-rich plasma in the treatment of chronic nonhealing leg ulcers. Plast Aesthet Res. 2014 Sep;1(2):65-69. [CrossRef]
12. Alexiadou K, Doupis J. Management of Diabetic Foot Ulcers. Diabetes Ther. 2012 Nov;3(1):4. [PubMed] [CrossRef]
13. Margolis DJ, Kantor J, Santanna J, Strom BL, Berlin JA. Effectiveness of platelet releasate for the treatment of diabetic neuropathic foot ulcers. Diabetes Care. 2001 Mar;24(3):483-8. [PubMed] [CrossRef]
14. El-Sharkawy H, Kantarci A, Deady J, Hasturk H, Liu H, Alshahat M, et al. Platelet-rich plasma: growth factors and pro- and anti-inflammatory properties. J Periodontol. 2007 Apr;78(4):661-9. [PubMed] [CrossRef]
15. Anitua E, Alonso R, Girbau C, Aguirre JJ, Muruzabal F, Orive G. Antibacterial effect of plasma rich in growth factors (PRGF®-Endoret®) against Staphylococcus aureus and Staphylococcus epidermidis strains. Clin Exp Dermatol. 2012 Aug;37(6):652-7. [PubMed] [CrossRef].

Received: 14 September 2016
Published online: 12 December 2016

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