Journal of IMAB - Annual Proceeding (Scientific Papers)
Publisher: Peytchinski, Gospodin Iliev
ISSN: 1312 773X (Online)
Issue: 2015, vol. 21, issue 4
Subject Area: Medicine - Gastroenterology
Pages: 969-973
DOI: 10.5272/jimab.2015214.969
Published online: 30 November 2015

J of IMAB 2015 Oct-Dec;21(4):969-973
Miglena Stamboliyska1Corresponding Autor, Violina Kaludova2, Milko Mirchev1, Iskren Kotzev1, Rosen Madjov3, Krasimir Metodiev2
1) Clinic of Gastroenterology, University Hospital ”St. Marina”, Medical University - Varna, Bulgaria
2) Department of preclinical and clinical sciences, Faculty of Pharmacy, Medical University - Varna, Bulgaria
3) Second Department of Abdominal Surgery, University Hospital ”St. Marina”, Medical University - Varna, Bulgaria.

Background: Unsuccessful treatment of H. pylori (HP) infection is constantly rising. Antibiotic susceptibility testing and selection of individualized therapy are expected to improve eradication of HP.
Aim: To assess the diagnostic accuracy of microbiology testing for HP and to assess the effectiveness of culture-guided susceptibility tested antibiotic therapy.
Materials and methods: A total of 62 HP infected patients, 35 with unsuccessful eradication and 27 treatment naive were subjected to culture testing and 3 endoscopy-based methods. In isolated НР colonies strain susceptibility and resistance were determined by disc-diffusion method. A triple therapy in the naïve group and quadruple in the unsuccessfully treated group was applied. The effectiveness of eradication was evaluated after 2 months by stool antigen test and/or urea breath test. Fisher’s exact test for comparison of therapeutic efficacy was used.
Results: НР colonies were isolated in 13 patients - 9 naïve and 4 unsuccessfully treated. Higher sensibility (100%) was shown towards UBT and high specificity between 95% - 100% compared to other methods. Its accuracy was 91.9%. In all patients with proven resistance culture-guided eradication was successful.
Conclusions: Microbiology and antibiotic susceptibility testing allows optimal diagnosis and therapy of HP infection.

Key words: Helicobacter pylori, eradication, microbiology testing, PCR, antibiotic susceptibility,

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Please cite this article in PubMed Style or AMA (American Medical Association) Style:
Stamboliyska M, Kaludova V, Mirchev M, Kotzev I, Madjov R, Metodiev K. Role and Importance of Microbiology testing in the Diagnosis and Treatment of Helicobacter pylori Infection. J of IMAB. 2015 Oct-Dec;21(4):969-973. doi:

Correspondence to: Assoc. prof. Miglena Stamboliyska, MD, PhD, Clinic of Gastroenterology, UMHAT St. Marina; 1, Hristo Smirnenski blvd., 9010 Varna, Bulgaria; E-mail:

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Received: 24 June 2015
Published online: 30 November 2015

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