Journal of IMAB - Annual Proceeding (Scientific Papers)
Publisher: Peytchinski, Gospodin Iliev
ISSN: 1312 773X (Online)
Issue: 2014, vol. 20, issue 5
Subject Collection: Oral and Dental Medicine
Pages: 610-616
DOI: 10.5272/jimab.2014205.610
Published online: 14 October 2014

J of IMAB 2014 Oct-Dec;20(5):610-616
Kamen KotsilkovCorresponding Autor, Christina Popova.
Department of Periodontology, Faculty of Dental Medicine, Medical University – Sofia, Bulgaria.

INTRODUCTION: Antibiotic use in chronic periodontitis may result in improvement in periodontal status, although many questions regarding the indications for this therapy remain unanswered. The polymicrobial etiology of the periodontal infection hinders the choice of the proper antibiotic agent. Furthermore the indiscriminate use of antibiotics could lead to high levels of resistance and to various adverse reactions.  In the recent years a various molecular diagnostics protocols were proposed in order to facilitate the decision for adjunctive antibiotic administration.
OBJECTIVE: The aim of this study is to compare the microbiological effectiveness of adjunctive antibiotic administration with the mechanical periodontal therapy.
METHODS: 30 patients with severe chronic periodontitis were enrolled in this study and were divided in 3 groups:
Control group – with mechanical debridement only.
Test group 1 – with combined adjunctive antibiotic administration using Amoxicillin+ Metronidazole.
Test group 2 – with target antibiotic administration according to the resuts from the Real Time PCR identification.
RESULTS: The prevalence of all the isolated microorganisms (exept. E.nodatum and C.gingivalis) in Test Group 2 demonstrates statistically significant reduction compared with the other treatment approaches. Almost complete elimination was registered for the consensus pathogens from the red and orange complexes (above 99% and 100% for P.intemedia).
CONCLUSION: The adjunct antibiotic treatment targeted with Real-Time PCR identification demonstrates almost complete elimination of the putative periodontal pathogens in the deep periodontal pockets in patients with severe chronic periodontitis. This result suggests slower recolonisation of these habitats thus limiting the risk for progression of the periodontal destruction.

Key words: severe chronic periodontitis, adjunctive antibiotic therapy, periodontal pathogens, Real Time PCR identification,

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Please cite this article in PubMed Style or AMA (American Medical Association) Style:
Kotsilkov K, Popova Ch. Real time PCR identification for target adjunctive antibiotic therapy of severe chronic periodontitis. Part II - microbiological effetivenes. J of IMAB. 2014 Oct-Dec;20(5):610-616. doi:

Correspondence to: Dr. Kamen Kotsilkov, Department of Periodontology, Faculty of Dental Medicine, Medical University – Sofia; 1, Sv. Georgi Sofiyski Blvd, 1431 Sofia, Bulgaria; E-mail:

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Received: 17 July 2014
Published online: 14 October 2014

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