back to 2013, vol. 19, b. 1
Journal of IMAB - Annual Proceeding (Scientific Papers)
Publisher: Peytchinski, Gospodin Iliev
ISSN: 1312 773X (Online)
Issue: 2013, vol. 19, book 1;
Subject Collection: Medicine
Page: 375-378
DOI: 10.5272/jimab.2013191.375
Published online: 1 February 2013

J of IMAB. 2013; 19(1):375-378;
Miroslav Marinov1, Iglika Marinova2, Christiana Romanova2, Nina Radeva3, Borislav Boychev4, M. Panteleeva5, Kosta Mirchev6, Radko Zl. Radev 1Corresponding Autor
1) Department of Preclinical and Clinical Sciences, 2) Department of Hygiene and disaster medicine, 3) Department of Health Management, 4) University Hospital – Varna, 5) Regional Health Inspectorate – Varna, 6) Department of Physiology and Pathophysiology,
Medical University – Varna, Bulgaria.

Purpose of the study is to track in, the blood flow and the metabolism in 15 organs of experimental rats, which are subjected to severe physical strain, treated with protein hydrolyzate and vitamine C (Vit. C). The animals are divided into one control and 4 experimental groups. After 30 minutes of swimming they are treated with already used produces, methionine 75selenium and and after another 2 hours of swimming, with 86rubidium. After being euthanized with thiopental and an autopsied, the following organs are taken from the rats: pancreas, spleen, testicles, duodenum, a part of small intestine and colon, adrenals, kidneys, liver, lungs, heart, aorta, a piece of muscle, part of a brain and stomach. Percentage of the activity per gram of tissue is determined, of the total activity, of the two isotopes for every organ and them being compared. Physical strain deteriorates blood flow and reduces the accumulation of methionine 75selenium, while the treatment with the hydrolyzate and Vit. C has a beneficial effect in almost all organs.

Key words: physical strain; methionine 75selenium; 86rubidium; protein hydrolysate; vitamine C.

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Please cite this article as:
Marinov M, Marinova I, Romanova Ch, Radeva N, Boychev B, Panteleeva M, Mirchev K, Radev RZ. Distribution of 86rubidium and metionine 75selenium in organs of rats after exercise and treatment with protein hydrolyzate and vitamin C. J of IMAB. 2013; 19(1):375-378. DOI: 10.5272/jimab.2013191.375.

Correspondence to: Radko Zlatkov Radev, MD, PhD. Department of preclinical and clinical medicine, Medical University of Varna, Bulgaria.

1. Barrow HM, McGee R, Tritschler KA. Practical Measurement in Physical Education and Sport. (4th sub ed.) Philadelphia, Pa: Lea & Febiger. 1989.
2. Karvonen J, Lemon PWR, Iliev I, & ed. Medicine in Sports Training and Coaching. Basel, Switzerland: Karger. 1992.
3. Hall JE. Guyton and Hall Textbook of Medical Physiology. 12th ed. Philadelphia, Pa: WB Saunders Company. 2010.
4. Kenney W.L, Wilmore JH, Costill DL. Physiology of Sport and Exercise. 5th ed. Champaign, IL: Human Kinetics. 2011.
5. Fletcher GF, Balady G, Blair SN, Blumenthal J, Caspersen C, Chaitman B, et al. Statement on exercise: benefits and recommendations for physical activity programs for all Americans. A statement for health professionals by the Committee on Exercise and Cardiac Rehabilitation of the Council on Clinical Cardiology, American Heart Association. Circulation. 1996 Aug 15;94(4):857-862. [PubMed]. [CrossRef].
6. Taylor NF, Dodd KJ, Shields N, Bruder A. Therapeutic exercise in physiotherapy practice is beneficial: a summary of systematic reviews 2002-2005. Aust J Physiother. 2007; 53(1):7-16. [PubMed].
7. Balady GJ, Williams MA, Ades PA, Bittner V, Comoss P, Foody JM, et al. Core components of cardiac rehabilitation/secondary prevention programs: 2007 update: a scientific statement from the American Heart Association Exercise, Cardiac Rehabilitation, and Prevention Committee, the Council on Clinical Cardiology; the Councils on Cardiovascular Nursing, Epidemiology and Prevention, and Nutrition, Physical Activity, and Metabolism; and the American Association of Cardiovascular and Pulmonary Rehabilitation. Circulation. 2007; 115 (20): 2675-2682. [PubMed] [Full Text].
8. Talbot LA, Morrell CH, Fleg JL, Metter EJ. Changes in leisure time physical activity and risk of all-cause mortality in men and women: The Baltimore Longitudinal Study of Aging. Prev Med. 2007; 45 (2-3): 169-76. [PubMed] [Full Text].
9. The President's Council on Physical Fitness and Sports. Physical activity and health. A report of the Surgeon General, US Department of Health and Human Services, Center for Disease Control and Prevention, National Center for Chronic Disease Prevention and Health Promotion. Centers for Disease Control and Prevention. Accessed 2007. Available at
10. American College of Sports Medicine. ACSM's Guidelines for Exercise Testing and Prescription. 8th ed. Philadelphia, Pa: Lippincott Williams & Wilkins; 2009.
11. American College of Sports Medicine. ACSM's Resource Manual for Guidelines for Exercise Testing and Prescription. 6th ed. Philadelphia, Pa: Lippincott, Williams & Wilkins; 2009.
12. Foster DO, Frydman ML. Comparison of microspheres and 86Rb+ as tracers of the distribution of cardiac output in rats indicates invalidity of 86Rb+-based measurements. Can J Physiol Pharmacol. 1978; 56 (1): 97-109. [PubMed]
13. Snyder DW, Doba N, Reis DJ. Regional distribution of blood flow during arterial hypertension produced by lesions of the nucleus tractus solitarii in rats. Circ Res. 1978; 42 (1): 87-91. [PubMed] [Full Text].
14. Williams LR, Leggett RW. The distribution of intracellular alkali metals in Reference Man. Phys Med Biol. 1987; 32 (2): 173-190. [PubMed]
15. Gillespie RB, Baumann PC, Singley CT. Dietary exposure of bluegills (Lepomis macrochirus) to (75) Se: uptake and distribution in organs and tissues. Bull Environ Contam Toxicol. 1988; 40(5):771-778. [PubMed]
16. Hayama E, Motoji N, Shigematsu A. The kinetics and dynamics of three kinds of radioactive methionine after i.v. administration in mice. Eur J Drug Metab Pharmacokinet. 1991; 16(4):287-297. [PubMed]
17. Schrauzer GN. The nutritional significance, metabolism and toxicology of selenomethionine. Adv Food Nutr Res. 2003; 47: 73-112. [PubMed]
18. Palavinskas R, Kriesten K, Schulten HR. Lithium, sodium, potassium and rubidium cation concentrations during the development of certain organs of unborn rabbits. Comp Biochem Physiol A Comp Physiol. 1984; 79(1):77-80. [PubMed]
19. Goresky CA, Simard A, Schwab AJ. Increased hepatocyte permeability surface area product for 86Rb with increase in blood flow. Circ Res. 1997; 80(5):645-654. [PubMed] [Full Text].
20. Franson JC, Hoffman DJ, Wells-Berlin A, Perry MC, Shearn-Bochsler V, Finley DL et al. Effects of dietary selenium on tissue concentrations, pathology, oxidative stress, and immune function in common eiders (Somateria mollissima). J Toxicol Environ Health A. 2007; 70(10):861-874. [PubMed].
21. Wilson FA, van den Borne JJ, Calder AG, O'Kennedy N, Holtrop G, Rees WD et al. Tissue methionine cycle activity and homocysteine metabolism in female rats: impact of dietary methionine and folate plus choline. Am J Physiol Endocrinol Metab. 2009; 296(4):E702-713. [PubMed] [Full Text].

Accepted for publication: 17 June 2012
Published online: 1 February 2013

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