back to 2012, vol. 18, b. 2
Journal of IMAB - Annual Proceeding (Scientific Papers)
Publisher: Peytchinski, Gospodin Iliev
ISSN: 1312 773X (Online)
Issue: 2012, vol. 18, book 2
Subject Collection: Oral and Dental Medicine
Pages: 191-199
DOI: 10.5272/jimab.2012182.191
Published online: 15 May 2012

J of IMAB 2012; 18(2):191-199
Natalia Gateva, Rossitza Kabaktchieva
Department of Pediatric dentistry, Faculty of Dental Medicine, Medical University, Sofia, Bulgaria.

Purpose: The aim this study is to compare the hybrid layer thickness and its micromorphological characteristics in samples from primary and permanent teeth following application of total etch adhesives.
Materials and methods: On intact specimens of 20 primary and 10 permanent teeth was created flat dentin surfaces. The patterns were divided in 6 groups. Two different total etch adhesive systems were used – one tree steps (OptiBond, Kerr) and one two steps (Exite, VivaDent). In groups 3, 4, 5 and 6 recommended etching time was used - 15 s, in groups 1 and 2 the etching time was reduced to 7 s. After applying the adhesive, resin composite build-ups were constructed. Thus restored samples are stored in saline solution for 24 hours at temperature 37 C. Then they are subjected to thermal stress in temperature between 5 C to 55 C for 1,500 cycles and to masticatory stress – 150,000 cycles with force 100 N in intervals of 0.4 s. After that the teeth are cut through the middle in medio-distal direction with a diamond disc. SEM observation was done to investigate the thickness of the hybrid layer and the presence of microgaps. Statistical analysis was performed with ANOVA and Tukey׳s tests.
Results: SEM observation showed significant differences of the hybrid layer thickness between primary and permanent teeth under equal conditions and after different etching time. Group 6 presented the highest average thickness 8.85 μ and group 1 the lowest average in hybrid layer 3.74 μ.
Conclusion: In primary teeth the hybrid layer thickness increases with the increased etching time. The hybrid layer thickness in primary teeth is greater than that of the hybrid layer in permanent teeth under equal conditions. For primary teeth it is more appropriate to reduce the etching time to 7s to obtain a hybrid layer with better quality

Key words: primary teeth, permanent teeth, hybrid layer, total etch adhesive, etching time.

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Please cite this article as: Gateva N, Kabaktchieva R. Hybrid layer Thickness in primary and permanent teeth - a comparison between total etch adhesives. J of IMAB. 2012; 18(2):191-199. doi: 10.5272/jimab.2012182.191

1. Bolaños-Carmona V, González-López S, Briones-Luján T, De Haro-Muñoz C, de la Macorra JC. Effects of Etching Time of Primary Dentin on Interface Morphology and Microtensile Bond Strength. Dent Mater. 2006 Dec;22(12):1121-1129. [PubMed ] [CrossRef ]
2. Bolaños-Carmona V, González-López S, De Haro-Muñoz C, Briones-Luján MT. Interfacial Morphology and Bond Strength of Self-Etching Adhesives to Primary Dentin With or Without Acid Etching. J Biomed Mater Res B Appl Biomater. 2008 Nov;87(2):499-507. [PubMed ] [CrossRef ]
3. Eick JD, Gwinnett AJ, Pashley DH, Robinson SJ. Current Concepts on Adhesion to Dentin. Crit Rev Oral Bio Med. 1997; 8(3): 306-335. [PubMed] [CrossRef]
4. Eliades G, Watts DS, Eliades T. Dental hard tissues and bonding. Interfacial phenomena and related properties. Springer 2005, 89-122
5. Gateva N. Comparative SEM examinations of alterations on dentine surface of primary and permanent teeth after etching. Problemi na dentalnata medizina 2008; 34(1): 71-84. (in Bulgarian)
6. Hals E. Observation on Giant Tubules in Human Coronal Dentin by Light Microscopy and Microradiography. Scand J Dent Res. 1983 Feb;91(1):1-7 [PubMed]
7. Hashimoto M, Ohno H, Endo K, Kaga M, Sano H, Oguchi H. The Effect of Hybrid Layer Thickness on Bond Strength: Demineralized Zone of Hybrid Layer. Dent Mater. 2000 Nov;16(6):406-411. [PubMed]
8. Hashimoto M, Ohno H, Kaga M, Sano H, Tay FR, Oguchi H, et al: Over-etching Effects on Micro-tensile Bond Strength and Failure Patterns for Two Dentin Bonding Systems. J Dent. 2002 Feb-Mar;30(2-3):99-105. [PubMed]
9. Hosoya Y, Marshall SJ, Watanabe LG, Marshall GW. Microhardness of Carious Deciduous Dentine. Oper Dent 2000 Mar-Apr;25(2):81-89. [PubMed]
10. Koutsi V, Noonan RG, Horner JA, Simpson MD, Matthews WG, Pashley DH. The effect of Dentin Depth on the Permeability and Ultrastructure of Primary Molars. Pediatr Dent. 1994 Jan-Feb;16(1):29-35. [PubMed]
11. Lohbauer U, Nikolaenko SA, Petschelt A, Frenkenberger R. Resin Tags do not Contribute to Dentin Adhesion in Self-etching Adhesives. J Adhes Dent. 2008 Feb;10(2):97-103. [PubMed]
12. Lopez GC, Baratieri VLN, Caldeira de Andrada MA, Vieira LCC. Dental adhesion: Present state of the art and future perspectives. QuintessenceInt. 2002 Mar;33(3):213-224. [PubMed]
13. Mahoney E., Holt A., Swain M., Kilpatric N.: The Hardess and Modulus of Elasticity of Primary Molar Teeth: an Ultra-micro-indentation Study. J Dent. 2000 Nov;28(8):589-594. [PubMed] [CrossRef]
14. Marquezan M, da Silveira BL, Burnett LH Jr, Rodrigues CR, Kramer PF. Microtensile Bond Strength of Contemporary Adhesives to Primary Enamel and Dentin. J Clin Pediatr Dent. 2007 Winter;32(2):127-132. [PubMed]
15. Marshall GW Jr, Marshall SJ, Kinney JH, Balooch M. The Dentin Substrate: Structure and Properties Related to Bonding. J Dent. 1997 Nov;25(6):441-458. [PubMed] [CrossRef]
16. Monksgaard EC. Wet or Dry, Normal or Deproteinized Dentin Surface as Substrate for Dentin Adhesives. Acta Odontol Scand. 2002 Jan;60(1):60-64. [PubMed]
17. Nakabayashi N., Kojima K., Masuhara E.: The promotion of adhesion by the infiltration of monomers into tooth substrates. J Biomed Mater Res. 1982 May;16(3):265-273. [PubMed]
18. Nör JE, Feigal RG, Dennison JB, Edwards CA. Dentin Bonding: SEM Comparison of the Resin-Dentin Interface in Primary and Permanent Teeth. J Dent Res. 1996 Jun;75(6):1396- 1403. [PubMed]
19. Nör JE, Feigal RG, Dennison JB, Edwards CA. Dentin Bonding: SEM Comparison of the Dentin Surface in Primary and Permanent Teeth. Pediatr Dent. 1997 May-Jun;19(4);246-252. [PubMed]
20. Osorio R, Aguilera FS, Otero PR, Romero M, Osorio E, Garcıa-Godoy F, Toledano M. Primary dentin etching time, bond strength and ultra-structure characterization of dentin surfaces. J  Dent. 20 10 Mar;38(3):222-231. [PubMed] [CrossRef]
21. Pashley DH, Ciucchi B, Sano H, Carvalho RM, Russell CM. Bond strength versus dentine structure: A modelling approach. Arch Oral Biol. 1995 Dec;40(12):1109-1118. [PubMed] [CrossRef]
22. Perdigão J., Lopes M.: The Effect of Etching Time on Dentin Demineralization. Quintessence International 2001; 32: 19-26
23. Pioch T, Garsia-Godoy F, Duschner H, Koch MJ, Staehle HJ, Dörfer CE. Effect of cavity preparation instruments (oscillating or rotating) on the composite-dentin interface in primary teeth. Dental Materials 2003 Jun;19(4): 259-263. [PubMed] [CrossRef]
24. Rauch RU, Losche GM, Rosansky J, Roulet JF. Comparison of quantitative margin analysis and dye penetration. Journal of  Dental Research 1996; 75: abstr.187; 41
25. Sano H, Shono T, Takatsu T, Hosoda H. Microporous Dentin Zone Beneath Resin-Impregnated Layer. Oper Dent. 1994 Mar-Apr;19(2):59-64. [PubMed]
26. Sardella TN, de Castro FL, Sanabe ME, Hebling J. Shortening of Primary Dentin Etching Time and its Implication on Bond Strength. J Dent. 2005 May;33(5):355-362. [PubMed] [CrossRef]
27. Shashikiran ND, Gunda S, Subba Reddy VV. Comparison of Resin-Dentin Interface in Primary and Permanent Teeth for Three Different Durations of Dentin Etching. J Indian Soc Pedod Prev Dent. 2002 Dec;20(4):124-131. [PubMed]
28. Spencer P, Wang Y, Walker MP, Swafford JR. Molecular Structure of Acid-Etched Dentin Smear Layers – in situ Study. J Dent Res. 2001 Sep;80(9):1802-1807. [PubMed] [CrossRef]
29. Spreafico D, Semeraro S, Mezzanzanica D, Re D, Gagliani M, Tanaka T, et al. The effect of air-blowing step on the technique sensitivity of four different adhesive systems. J Dent. 2006 Mar;34(3):237-244. [PubMed] [CrossRef]
30. Städtler P: Dentinhaftmittel. Stomatologie 2007; 104(4): 101–109. [CrossRef]
31. Tulunoglu O, Tulunoglu I. Resin-dentin interfacial morphology and shear bond strengths to primary dentin after long-term water storage: An in vitro study. Quintessence Int. 2008 May;39(5): 427-437. [PubMed]
32. Uekusa S, Yamaguchi K, Miyazaki M, Tsubota K, Kurokawa H, Hoyosa Y. Bonding efficacy of single-step self-etch systems to sound primary and permanent tooth dentin. Oper Dent. 2006 Sep-Oct;31(5):569–576. [PubMed] [CrossRef]
33. Van Meerbeek B, Conn LJ Jr, Duke E, Eick J, Robinson S, Guerrero D. Correlative transmission electron microscopy examination of nondemineralized and demineralized rezin-dentin interfaces formed by two dentin adhesive systems. J Dent Res. 1996 Mar;75(3):879-888. [Pubmed] [CrossRef]
34. Van Meerbeek B, De Munck J, Yoshida Y, Inoue S, Vargas M, Vijay P, et al. Buonocore memorial lecture. Adhesion to enamel and dentin: current status and future challenges. Oper Dent. 2003 May-Jun;28(3):215-235. [PubMed]
35. Van Meerbeek B, Inokoshi S, Braem M, Lambrechts P, Vanherle G. Morphological aspects of the resin–dentin interdiffusion zone with different dentin adhesive systems. J Dent Res. 1992 Aug;71(8):1530–1540. [PubMed] [CrossRef]
36. Van Meerbeek B, Van Landuyt K, De Munck JD, Hashimoto M, Peumans M, et al. Technique-Sensitivity of Contemporary Adhesives. Dent Mater J. 2005 Mar;24(1):1-13. [PubMed]
37. Van Meerbeek B.,Vargas M., Inoue S., Yoshida Y., Peumans M Lambrechts P. et all.: Adhesives and cements to promote preservation dentistry. Operative Dentistry 2001; 6: 119-144
38. Wang Y, Spencer P. Analysis of Acid-Treated Dentin Smear Debris and Smear Layers Using Confocal Raman Microspectroscopy. J Biomed Mater Res. 2002 May;60(2):300-308. [PubMed]
39. Yaseen SM, Subba Reddy VV. Comparative evaluation of shear bond strength of two self-etching adhesives (sixth and seventh generation) on dentin of primary and permanent teeth: An in vitro study. J Indian Soc Pedod Prev Dent. 2009 Jan-Mar;27(1):33-38. [PubMed] [CrossRef]

Accepted for publication: 14 February 2012
Issue published online: 15 May 2012

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