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Publisher: Peytchinski Publishing
ISSN: 1312-773X (Online)
Issue: 2018, vol. 24, issue1
Subject Area: Dental Medicine
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DOI: 10.5272/jimab.2018241.1914
Published online: 01 March 2018
Original article
J of IMAB. 2018 Jan-Mar;24(1):1914-1917
INFLUENCE OF STORAGE MEDIA ON THE VITALITY OF STEM CELLS FROM APICAL PAPILLA
Krasimir Hristov1
, Nataliya Gateva1, Pavel Stanimirov2, Nikolay Ishkitiev3, Rozaliya Tsikandelova3,
1) Department of Pediatric Dentistry, Faculty of Dental Medicine, Medical University of Sofia, Sofia, Bulgaria.
2) Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Medical University of Sofia, Sofia, Bulgaria.
3) Department of Chemistry and Biochemistry, Faculty of Medicine, Medical University of Sofia, Sofia, Bulgaria.
ABSTRACT:
Purpose: Immediate replantation is the treatment of choice for avulsed permanent teeth. Transportation of avulsed teeth to the dentist office requires storage in suitable media, thus keeping periodontal and periapical tissues viable. Effect of different storage media on periodontal cells vitality has been thoroughly researched, but there is no available data concerning SCAP. The purpose of this study is to evaluate the storage media effect on SCAP vitality.
Materials and methods: Our study includes 10 third molars of 14-17 years old patients, extracted due to orthodontic reasons. The apical papilla of those teeth is handled according to protocol, and the cell growth is maintained in DMEM. Cells are divided into 4 groups: 1-positive control group, 2- SOS Dentobox, 3 - (HBSS), 4 - negative control group. A colourimetric assay was used to determine the vital cell count in each group.
Results: Signal strength of the cells, stored in SOS Dentobox, is on average 30% weaker than the positive control group cells’ signal, while the signal strength of the cells, stored in HBSS, is 65,2% weaker.
Conclusion: SOS Dentobox provides better SCAP cell vitality than HBSS
Keywords: stem cells from apical papilla, storage media, avulsion,
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Please cite this article in PubMed Style or AMA (American Medical Association) Style:
Hristov K, Gateva N, Stanimirov P, Ishkitiev N, Tsikandelova R. Influence of Storage Media on the Vitality of Stem Cells from Apical Papilla. J of IMAB. 2018 Jan-Mar;24(1):1914-1917.
DOI: 10.5272/jimab.2018241.1914
Correspondence to: Krasimir Hristov , Department of Pediatric Dentistry, Faculty of Dental Medicine, Medical University of Sofia; 1, St. Georgi Sofiyski str., 1431 Sofia, Bulgaria; E-mail: dr.hristov12@gmail.com
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Received: 27 November 2017
Published online: 01 March 2018