head JofIMAB
Journal of IMAB - Annual Proceeding (Scientific Papers)
Publisher: Peytchinski Publishing
ISSN: 1312-773X (Online)
Issue: 2018, vol. 24, issue1
Subject Area: Dental Medicine
DOI: 10.5272/jimab.2018241.1914
Published online: 01 March 2018

Original article

J of IMAB. 2018 Jan-Mar;24(1):1914-1917
Krasimir Hristov1ORCID logo Corresponding Autoremail, Nataliya Gateva1ORCID logo, Pavel Stanimirov2ORCID logo, Nikolay Ishkitiev3ORCID logo, Rozaliya Tsikandelova3ORCID logo,
1) Department of Pediatric Dentistry, Faculty of Dental Medicine, Medical University of Sofia, Sofia, Bulgaria.
2) Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Medical University of Sofia, Sofia, Bulgaria.
3) Department of Chemistry and Biochemistry, Faculty of Medicine, Medical University of Sofia, Sofia, Bulgaria.

Purpose: Immediate replantation is the treatment of choice for avulsed permanent teeth. Transportation of avulsed teeth to the dentist office requires storage in suitable media, thus keeping periodontal and periapical tissues viable. Effect of different storage media on periodontal cells vitality has been thoroughly researched, but there is no available data concerning SCAP. The purpose of this study is to evaluate the storage media effect on SCAP vitality.
Materials and methods: Our study includes 10 third molars of 14-17 years old patients, extracted due to orthodontic reasons. The apical papilla of those teeth is handled according to protocol, and the cell growth is maintained in DMEM. Cells are divided into 4 groups: 1-positive control group, 2- SOS Dentobox, 3 - (HBSS), 4 - negative control group. A colourimetric assay was used to determine the vital cell count in each group.
Results: Signal strength of the cells, stored in SOS Dentobox, is on average 30% weaker than the positive control group cells’ signal, while the signal strength of the cells, stored in HBSS, is 65,2% weaker.
Conclusion: SOS Dentobox provides better SCAP cell vitality than HBSS

Keywords: stem cells from apical papilla, storage media, avulsion,

pdf - Download FULL TEXT /PDF 599 KB/
Please cite this article in PubMed Style or AMA (American Medical Association) Style:
Hristov K, Gateva N, Stanimirov P, Ishkitiev N, Tsikandelova R. Influence of Storage Media on the Vitality of Stem Cells from Apical Papilla. J of IMAB. 2018 Jan-Mar;24(1):1914-1917.
DOI: 10.5272/jimab.2018241.1914

Corresponding AutorCorrespondence to: Krasimir Hristov , Department of Pediatric Dentistry, Faculty of Dental Medicine, Medical University of Sofia; 1, St. Georgi Sofiyski str., 1431 Sofia, Bulgaria; E-mail: dr.hristov12@gmail.com

1. Fontain SB, Camp JH. Traumatic injuries. In: Pathways of the pulp. Еdited by Cohen S, Richard BC. 6th edition. Mosby. April 1994. Chapter 16: p.436-437.
2. Andreasen JO, Borum MK, Jacobsen HL, Andreasen FM. Replantation of 400 avulsed permanent incisors. Factors related to pulpal healing. Endod Dent Traumatol. 1995 Apr;11(2):59-68. [PubMed]
3. Andersson L, Bodin I. Avulsed human teeth replanted within 15 minutes--a long-term clinical follow-up study. Endod Dent Traumatol. 1990 Feb;6(1):37-42. [PubMed] [CrossRef]
4. King M, Cvek M, Mejare I. Rate and predictability of pulp revascularization in therapeutically reimplanted permanent incisors. Endod Dent Traumat. 1986 Jun;2(3):83-89. [PubMed]
5. Bazmi BA, Singh AK, Mubtasum H, Kar S. Storage media for avulsed tooth – a review. Indian J of Multidiscip Dent. 2013 May-Jul;3(3):741-49.
6. Martin MP, Pileggi R. A quantitative analysis of Propolis: a promising new storage media following avulsion. Dent Traumatol. 2004 Apr;20(2):85-90.
7. Moazami F, Mirhadi H, Geramizadeh B, Sahebi S. Comparison of soymilk, powdered milk, Hank’s balanced salt solution and tap water on periodontal ligament cell survival. Dent Traumatol. 2012 Apr;28(2):132-5. [PubMed] [CrossRef]
8. Khademi AA, Saei S, Mohajeri MR, Mirkheshti N, Ghassami F, Torabi nia N, et al. A new storage medium for an avulsed tooth. J Contemp Dent Pract. 2008 Sep;9(6):25-32. [PubMed]
9. Al-Haj Ali SN, Al-Jundi S, Mhaidat N. Comparison of Coconut Water and Jordanian Propolis on Survival of Bench-dried Periodontal Ligament Cells: An in vitro Cell Culture Study. Int J Clin Pediatr Dent. 2013 Sep;6(3):161-165. [PubMed] [CrossRef]
10. Bhagyshree T, Ajinkya PM. Storage media used for avulsed teeth - the first aid. EJPMR. 2017 Feb;4(2):433-437.
11. Is Khinda V, Kaur G, Brar G, Kallar S, Khurana H. Clinical and Practical Implications of Storage Media used for Tooth Avulsion. Int J Clin Pediatr Dent. 2017 Apr-Jun;10(2):158-65. [PubMed] [CrossRef]
12. Malhotra N. Current developments in interim transport (storage) media in dentistry: an update. Br Dent J. 2011 Jul;211(1):29-33. [PubMed] [CrossRef]
13. Cvek M, Cleaton-Jones P, Austin J, Lownie J , Kling M, Fatti P. Pulp revascularization in reimplanted immature monkey incisors – Predictability and the effect of antibiotic systemic prophylaxis. Endod Dent Traumatol. 1990 Aug;6(4):157-69. [PubMed]
14. Andersson L, Andreasen JO, Day P, Heithersay G, Trope M, Diangelis AJ, Kenny DJ, et al. Guideline for the Management of Traumatic Dental Injuries: 2. Avulsion of Permanent Teeth. Dent Traumatol. 2012 Apr;28(2):88-96. [PubMed]
15. Barrett EJ, Kenny DJ. Avulsed permanent teeth: A review of the literature and treatment guidelines. Endod Dent Traumatol. 1997 Aug;13(4):153-63. [PubMed]
16. Andreasen JO, Borum MK, Jacobsen HL, Andreasen FM. Replantation of 400 avulsed permanent incisors: 4. Factors related to periodontal ligament healing. Endod Dent Traumatol. 1995 Apr;11(2):76-89. [PubMed]
17. Hiltz J, Trope M. Vitality of human lip fibroblasts in milk, Hank’s Balanced Salt Solution, and Viaspan storage media. Endod Dent Traumatol. 1991 Apr;7(2):69-72. [PubMed]
18. Trope M, Friedman S. Periodontal healing of replanted dog teeth stored in Viaspan, milk and Hank's balanced salt solution. Endod Dent Traumatol. 1992 Oct;8(5):183-8. [PubMed]
19. Blomlof L, Otteskog P, Hammarstrom L. Effect of storage in media with different ion strengths and osmolalities on human periodontal ligament cells. Scand J Dent Res. 1981 Apr;89(2):180-7. [PubMed]
20. Udoye CI, Jafarzadeh H, Abbott PV. Transport media for avulsed teeth: A review. Aust Endod J. 2012 Dec;38(3):129-36. [PubMed] [CrossRef]
21. Pohl Y, Tekin U, Boll M, Filippi A, Kirschner H. Investigations on a cell culture medium for storage and transportation of avulsed teeth. Aust Endod J. 1999 Aug;25(2):70-5. [PubMed]
22. Huang GT, Sonoyama W, Liu Y, Liu H, Wang S, Shi S. The hidden treasure in apical papilla: the potential role in pulp/dentin regeneration and bioroot engineering. J Endod. 2008 Jun;34(6):645-51. [PubMed] [CrossRef]
23. Chrepa V, Pitcher B, Henry M, Diogenes A. Survival of the Apical Papilla and Its Resident Stem Cells in a Case of Advanced Pulpal Necrosis and Apical Periodontitis. J Endod. 2017 Apr;43(4):561-567. [CrossRef].

Received: 27 November 2017
Published online: 01 March 2018

back to Online Journal