Journal of IMAB - Annual Proceeding (Scientific Papers)
Publisher: Peytchinski, Gospodin Iliev
ISSN: 1312 773X (Online)
Issue: 2016, vol. 22, issue 2
Subject Area: Dental Medicine
Pages: 1127-1131
DOI: 10.5272/jimab.2016222.1127
Published online: 31 May 2016

J of IMAB 2016 Apr-Jun;22(2):1127-1131
Biljana Ilkovska1 Corresponding Autor, Bisera Kotevska2, Georgi Trifunov2, Branimir Kanazirev3.
1) Department of Medical Biochemistry, PHO Clinical Hospital Bitola, Macedonia
2) Department of Dermatology and Venereology and Otorhinolaryngology, Tokuda Hospital, Sofia, Bulgaria
3) Department of Medicine, Medical University, Varna, Bulgaria.

Background: Hepcidin has emerged as the central regulatory molecule of iron homeostasis. Iron deficiency and iron overload play a major role in molecular insights of many disease states and serum hepcidin normal values and biochemical correlations are of substantial importance.
Objective: The aim of this study is to examine the serum hepcidin reference range, gender and age differences, menopausal dependence and biochemical correlates in healthy subjects.
Methods: Serum hepcidin concentration was measured with a competitive enzyme-linked immunosorbent assay (DRG Hepcidin-25 ELISA Kit) together with hemoglobin, hematocrit, serum iron, transferrin and C-reactive protein in 120 healthy subjects both men and pre- and post-menopausal women.
Results: Normal serum hepcidin values were found in the range of 1,23 – 36,46 ng/mL (mean 9,25 ± 6,45 ng/mL).There were statistically significant differences in measured hepcidin levels between men  (12,34 ± 7,37 ng/mL) and women (6,16 ± 3,2 ng/mL) (p<0.01) and between pre- menopausal (5,51 ± 2,8 ng/mL) and post-menopausal women (7,29 ± 3,59 ng/mL) ( p<0,05). Strong correlations were found with serum ferritin and hemoglobin but not with serum iron, transferrin and CRP. No 5-year age interval differences were deemed significant.
Conclusion: Serum hepcidin concentration varied substantially between subjects, which is reflected in wide reference ranges. Serum hepcidin levels were gender and menopausal status related and were in correlation with hemoglobin and serum ferritin in healthy subjects.

Key words: hepcidin, range, gender, menopause, healthy subjects,

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Please cite this article in PubMed Style or AMA (American Medical Association) Style:
Ilkovska B, Kotevska B, Trifunov G, Kanazirev B. Serum hepcidin reference range, gender differences, menopausal dependence and biochemical correlates in healthy subjects. J of IMAB. 2016 Apr-Jun;22(2):1127-1131. DOI:

Correspondence to: Biljana Ilkovska, Department of Medical Biochemistry PHO Clinical Hospital dr. Trifun Panovski; st. Partizanska b.b, 7000 Bitola, R. Macedonia; E-mail:

1. Kroot JJ, Tjalsma H, Fleming RE, Swinkels DW. Hepcidin in Human Iron Disorders: Diagnostic Implications. Clin Chem. 2011 Dec;57(12):1650-1669. [PubMed] [CrossRef]
2. Itkonen O, Stenman UH, Parkkinen J, Soliymani R, Baumann M, Hämäläinen E. Binding of hepcidin to plasma proteins. Clin Chem.  2012 Jul;58(7):1158–1160. [PubMed] [CrossRef]
3. Campostrini N, Traglia M, Martinelli N, Corbella M, Cocca M, Manna D, et al. Serum levels of the hepcidin-20 isoform in a large general population: the Val Borbera study. J Proteomics. 2012 Dec;76 Spec No:28-35. [PubMed] [CrossRef]   
4. Pigeon C, Ilyin G, Courselaud B, Leroyer P, Turlin B, Brissot P, et al. A new mouse liver-specific gene, encoding a protein homologous to human antimicrobial peptide hepcidin, is overexpressed during iron overload. J Biol Chem. 2001 Mar;276(11):7811-7819. [PubMed] [CrossRef]
5. Rishi G, Wallace DF, Subramaniam VN. Hepcidin: regulation of the master iron regulator. Biosci Rep. 2015 Jun;35(3):e00192. [PubMed] [CrossRef]
6. Theurl I, Aigner E, Theurl M, Nairz M, Seifert M, Schroll A, et al. Regulation of iron homeostasis in anemia of chronic disease and iron deficiency anemia: diagnostic and therapeutic implications. Blood. 2009 May; 113(21):5277-5286. [PubMed] [CrossRef]
7. Swinkels DW, Wetzels JF. Hepcidin: a new tool in the management of anaemia in patients with chronic kidney disease?& Nephrol Dial Transplant. 2008 Aug;23(8>):2450-2453. [PubMed] [CrossRef]
8. Galesloot TE, Vermeulen SH, Geurts-Moespot AJ, Klaver SM, Kroot JJ, van Tienoven D, et al. Serum hepcidin: reference ranges and biochemical correlates in the general population. Blood. 2011 Jun;117(25):e218-25. [PubMed] [CrossRef]
9. Geerts I, Vermeersch P, Joosten E. Evaluation of the first commercial hepcidin ELISA for the differential diagnosis of anemia of chronic disease and iron deficiency anemia in hospitalized geriatric patients. ISRN Hematol. 2012; 2012:567491. [PubMed] [CrossRef]
10. Аshby DR, Gale DP, Busbridge M, Murphy KG, Duncan ND, Cairns TD, et al. Plasma hepcidin levels are elevated but responsive to erythropoietin therapy in renal disease. Kidney Int. 2009 May;75(9):976-981. [PubMed] [CrossRef]
11. Ganz T, Olbina G, Girelli D, Nemeth E, Westerman M. Immunoassay for human serum hepcidin. Blood. 2008 Nov;112(10):4292-7. [PubMed] [CrossRef]
12. Roe MA, Collings R, Dainty JR, Swinkels DW, Fairweather-Tait SJ. Plasma hepcidin concentrations significantly predict interindividual variation in iron absorption in healthy men. Am J Clin Nutr. 2009 Apr;89(4):1088-91. [PubMed] [CrossRef].

Received: 18 March 2016
Published online: 31 May 2016

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