Journal of IMAB - Annual Proceeding (Scientific Papers)
Publisher: Peytchinski, Gospodin Iliev
ISSN: 1312 773X (Online)
Issue: 2014, vol. 20, issue 3
Subject Collection: Oral and Dental Medicine
Pages: 533-536
DOI: 10.5272/jimab.2014203.533
Published online: 18 July 2014

J of IMAB 2014 Jul-Sep;20(3):533-536
Mirela Marinova-Takorova1 Corresponding Autor, Radostina Anastasova2, Vladimir E. Panov2.
1) Department of Conservative Dentistry, Faculty of Dental Medicine, Medical University - Sofia, Bulgaria.
2) Department of Conservative dentistry and Oral pathology, Faculty of Dental medicine, Medical University - Varna, Bulgaria.

Purpose: The aim of the presented in vitro study was to evaluate the effectiveness of the device DIAGNOcam and the laser fluorescence device DIAGNOdent for early diagnosis of occlusal caries and to compare it with three traditional methods – visual and tactile, dye and radiographic examination.
Material and methods: The sample consisted of 60 extracted human teeth. Three clinicians diagnosed independently the presence or absence of early occlusal surface caries with the visible tactile method,DIAGNOcam andDIAGNOdent. Then X-rays were taken and the dentists viewed them separately, without magnification.Fuchsine was applied for 30 sec. and then washed under running water for 20 min. Places where dye was present were registered. Statistical analysis was performed with SPSS package of Windows.
Results: The results showed high level of correspondence between the authors for all the diagnostic methods.  Concerning different methods applied for caries diagnosis there was a correlation between the methods, but it was diverging for the different methods. No correlation between radiographic exam and data obtained with DIAGNOdent and dye staining was observed.
Conclusions: Based on the obtained results we may conclude that the least sensitive method for fissure caries diagnosis was the dye staining, followed by the radiographic exam. The results, obtained by DIAGNOdent and DIAGNOcam were very close, but DIAGNOcams’ data was better correlating with the clinical results.DIAGNOcam procedure can be judged as equivalent in the detection of occlusal dentine lesions when compared to clinical results.

Key words: early caries diagnosis, laser fluorescence,

- Download FULL TEXT /PDF 722 KB/
Please cite this article in PubMed Style or AMA (American Medical Association) Style:
Marinova-Takorova M, Anastasova R, Panov VE. Comparative evaluation of the effectiveness of five methods for early diagnosis of occlusal caries lesions – in vitro study. J of IMAB. 2014 Jul-Sep;20(3):533-536. doi:

Correspondence to: Dr. Mirela Marinova-Takorova, Department of Conservative Dentistry, Faculty of Dental Medicine, Medical University - Sofia; 1, St. George Sofiiski Str., 1431 Sofia, Bulgaria; E-mail:

1. Frencken JE, Peters MS, Manton DJ, Leal SC, Gordan VV, Eden E. Minimal intervention dentistry (MID) for managing dental caries – a review: report of a FDI task group. Int Dent J. 2012 Oct;62(5):223-43. [PubMed] [CrossRef]
2. Kudiyirickal MG, Ivančaková R. Early enamel lesion. Part I. Classification and detection. ActaMedica (Hradec Kralove). 2008; 51(3):145-149. [PubMed]
3. Kudiyirickal MG, Ivančaková R. Early enamel lesion. Part II. Histo-morphology and prevention. ActaMedica (Hradec Kralove). 2008 Mar;51(3):151-6. [PubMed]
4. Amaechi BT. Emerging technologies for diagnosis of dental caries: The road so far. J Appl Phys. 2009 May;105:102047. [CrossRef]
5. Weerheijm KL, Kidd EA, Groen HJ. The effect of fluoridation on the occurrence of hidden caries in clinically sound occlusal surfaces. Car Res. 1997; 31(1):30-34. [PubMed]
6. McComb D, Tam LE. Diagnosis of occlusal caries: Part I. Conventional methods. J Can Dent Assoc. 2001 Sep;67(8):454-457. [PubMed]
7. Karlsson L. Caries detection methods based on changes in optical properties between healthy and carious tissue. Int J Dent. 2010; 2010:270729. [PubMed] [CrossRef]
8. Stookey GK, Gonzalez-Cabezas C. Emerging methods of caries diagnosis. J Dent Educ. 2001 Oct; 65(10): 1001-6. [PubMed]
9. Bahrololoomi Z, Musavi SA, Kabudan M. In vitro evaluation of the efficacy of laser fluorescence (DIAGNOdent) to detect demineralization and remineralization of smooth enamel lesions. J Conserv Dent. 2013 Jul;16(4):362-366. [PubMed] [CrossRef].
10. Mendes FM, Nicolau J, Duarte DA. Evaluation of the effectiveness of laser fluorescence in monitoring in vitro remineralization of incipient caries lesions in primary teeth. Car Res. 2003 Nov-Dec;37(6):442-444. [PubMed] [CrossRef]
11. Diniz MB, Paes Leme AF, Kardoso KdeS, Rodrigues JdeA, Corderio RdeC. The efficacy of laser fluorescence system to detect in vitro demineralization and remineralization of smooth enamel surfaces. Photomed Laser Surg 2009 Feb;27(1):57-61. [PubMed] [CrossRef]
12. Astvaldsdóttir A, Tranæus S, Karisson L, Peter Holbrook W. DIAGNOdent measurements of cultures of selected oral bacteria and demineralized enamel. Acta Odontil Scand. 2010 May;68(3):148-153. [PubMed] [CrossRef]
13. Ricketts D. The eyes have it. How good is DIAGNOdent at detecting caries? Evid Based Dent. 2005; 6(3):64-65. [PubMed] [CrossRef]

Received: 29 April 2014
Published online: 18 July 2014

back to Online Journal