back to 2012, vol. 18, b. 3
Journal of IMAB - Annual Proceeding (Scientific Papers)
Publisher: Peytchinski, Gospodin Iliev
ISSN: 1312 773X (Online)
Issue: 2012, vol. 18, book 3;
Subject Collection: Medicine
Page: 315-322
DOI: 10.5272/jimab.2012183.315
Published online: 09 November 2012

J of IMAB. 2012; 18(3):315-322
Asparuh G. Nikolov1, Georgi Nicoloff2, Ivan Tsinlikov1,  Ivanka Tsinlikova1
1) Department of Propedeutics of Internal Diseases, Medical University, Pleven
2) Division of Biology, Medical University, Pleven, Bulgaria.

Background and Aims: Thickening of basement membrane in capillaries and small vessels is a well-known finding and important in the progression of diabetic microangiopathy. Patients with diabetes mellitus and arterial hypertension are at higher risk of vascular disease.
Material and methods: To monitor the metabolism of the basement membrane protein collagen type IV (CIV) in type 2 diabetes mellitus (T2DM), serum levels of antibodies to CIV (ACIV) IgG, IgM and IgA were measured using an ELISA method in 93 patients with type 2 diabetes mellitus and arterial hypertension (AH) (mean age 61,4±11,3 years, diabetes duration 9,88±3,12 years; hypertension duration 9,28±4,98). These values were compared to serum antibodies to CIV in 42 age and sex matched controls. Diabetics were divided in two groups according to presence- Group 1 (n=67) or absence- Group 2 (n=26) of microangiopathy.
Results: Patients with T2DM and AH showed statistically significant higher levels of ACIV IgG  in comparison to healthy controls (0.30±0.12 vs. 0.21±0.08) (p=0.0001). Group 1 showed significantly hihger levels of  ACIV IgG  than Group 2 (0.32±0.13vs. 0.24±0.08) (p=0.009) and healthy controls (0.32±0.13vs. 0.21±0.08) (p=0.0001). ACIV IgG are statistically significant higher in diabetics with retinopathy than this without (0.33±0.10 vs. 0.26±0.13) (р=0.04). ACIV IgG correlates with diabetes duration (r=0.49); (p=0.0004), retinopathy (r=0.20); (p=0.05) and BMI (r=-0.24); (p=0.05). Serum ACIV IgM and IgA levels in patients with T2DM and AH were lower than these in controls, but the differences are not statistically significant.
Conclusion: Our study showed a relationship between elevation of  serum levels of ACIV IgG in diabetics and development of microangiopathy.

Key words: Diabetes mellitus, arterial hypertension, anti-collagen type IgG, micrangiopathy.

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Please cite this article as: Nikolov AG, Nicoloff G, Tsinlikov I, Tsinlikova I. ANTI-COLLAGEN TYPE IV ANTIBODIES AND THE DEVELOPMENT OF MICROVASCULAR COMPLICATIONS IN DIABETIC PATIENTS WITH ARTERIAL HYPERTENSION. J of IMAB. 2012; 18(3):315-322. doi: 10.5272/jimab.2012183.315.

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Accepted for publication: 18 August 2012
Issue published online: 09 November 2012

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